Intertest® Avian PPD Tuberculin

Intertest® Avian PPD Tuberculin is used for diagnosis of avian tuberculosis and comparative tuberculin test in cattle and other domestic animals.


Intertest® Avian PPD Tuberculin
Reg. No. G3754 (Act 36/1947)
Namibia Reg. No. V07/24.9/374 NS0

Intertest® Avian PPD Tuberculin is used for diagnosis of avian tuberculosis and comparative tuberculin test in cattle and other domestic animals.NOTE
Tuberculosis is a controlled disease in terms of the Animal Diseases Act (Act 35/1984). In terms of the act it is stipulated that:

  1. Any occurrence or suspected occurrence of tuberculosis has to be reported immediately to the local State Veterinarian.
  2. A tuberculin test may be carried out only by a state veterinary officer, an authorised person or a veterinarian. All tuberculin test results have to be communicated in writing to the State Veterinarian who is responsible for the determination of the disease status of an animal with regards to tuberculosis.
  3. Infected, suspect and contact animals shall be isolated, tested, marked and slaughtered as determined by the State Veterinarian.

Intertest® Avian PPD Tuberculin is a purified protein derivative (PPD) of Mycobacterium avium strain D4 cultures. It contains no live bacteria.
1 mℓ of the solution contains 25 000 IU and a maximum of 0,5 % of phenol as preservative.STORAGE

  • Store between 2 °C and 8 °C.
  • Do not freeze.
  • Protect from light.
  • Do not use the product after the expiry date.
  • After broaching the vial, use immediately.


  • Withdrawal period: None.
  • Acutely sick animals are to be exempted from administration of Intertest® Avian PPD Tuberculin.
  • In order to prevent inflammatory reactions the test should be administered aseptic.
  • In case of infections with mycobacteria of other species, non-specific reactions may result. In order to differentiate between avian tuberculosis and bovine tuberculosis a comparative tuberculosis test should be performed.
  • The comparative test for bovine tuberculosis should be administered at the earliest 6 weeks after previous administration of Intertest® Avian PPD Tuberculin to prevent false results. Similar waiting periods should be observed before a post-examination in poultry.
  • To prevent false negative results, animals which are treated, e.g. with corticosteroids or ACTH (Adrenocorticotropic hormone), are to be exempted from testing with Intertest® Avian PPD Tuberculin. After the administration of live viral vaccines false negative results cannot be ruled out.
  • On rare occasions latently infected animals or sensitised animals may show allergic or anaphylactic reactions when injected with Intertest® Avian PPD Tuberculin.
  • Although this product has been extensively tested under a large variety of conditions, failure thereof may ensue as a result of a wide range of reasons. If this is suspected, seek veterinary advice and notify the registration holder.

Although Intertest® Avian PPD Tuberculin does not contain living organisms, it is recommended to dispose of waste material derived from the product according to local waste disposal regulations.

One dose of 0,1 mℓ contains 2 500 IU.
The correct placement of the injection is indicated by a pea-like swelling at the site of injection.Comparative tuberculin test

  • In bovines with suspect results a comparative tuberculin test should be done with bovine and avian tuberculin simultaneously administered at the earliest 6 weeks after a previous administration of tuberculin.
  • The execution of the comparative tuberculin test follows in the same manner as the process of bovine tuberculin testing. The 2 intracutaneous injections should be undertaken either on one side (in that case bovine tuberculin above and avian tuberculin below) or on opposite sides of the body at corresponding positions (on the neck at the ridge of the shoulder blade). In the latter case the possible occurrence of clinical symptoms at the points of injection (specifically a parallel infection of the regional lymphatic nodes and lymphatic vessels) can be defined more clearly.
  • 0,1 mℓ of bovine tuberculin with 5 000 IU and 0,1 mℓ avian tuberculin with 2 500 IU should be injected into a fold of skin. The points of injection are to be marked. This can be accomplished most practically by shaving the skin in an area of approximately 8 to 10 cm in length and approximately 2 to 3 cm in width. In these areas no thickening should occur. At both points of injection the thickness of the fold of the skin is to be measured (e.g. Hauptner-calliper) and noted. It is important to see to it that the tuberculin is injected strictly intradermal and not subcutaneously. In addition care should be taken that no vaccine flows out of the injection site. In order to check the correct positioning of the intradermal injection, it has proven prudent to check the point of injection with the tip of the finger for the presence of a pea-like swelling.
  • The result of the tuberculin tests should not be read and evaluated earlier than 72 hours and not later than 96 hours after the injection of the tuberculin. During the reading, particular note should be taken of the careful measuring of the thickness of the skin at the point of injection. The evaluation of the tuberculin reaction follows through comparison of the reactions obtained with bovine and avian tuberculin. The evaluation is to be executed taking cognisance of the clinical symptoms as presented at the point of injection, i.e. pain, a doughy consistency, exudation and necrosis, particularly however on the basis of the actual precisely measured increase in thickness of the skin. An evaluation undertaken solely on the basis of palpation is in no way sufficient.

Interpretation of reactions
The interpretation of reactions shall be based on clinical observations and the recorded increase in skin-fold thickness at the sites of injection 72 hours after injection of tuberculin.

The following key is to be used as a guideline for evaluation:

Bovine minus
avian tuberculin
Thickness of skin
(difference in mm)
Positive resultMore than 4,0 mmPositive
2,1 to 4,0 mmSuspect
Up to 2,0 mmNegative
Negative resultIncrease which is less than or equal to the reaction at the site of avian injection and absence of clinical signs.Negative
  1. If clear clinical symptoms occur, even with a very small margin in bovine tuberculin, the presence of a bovine tuberculosis infection is possible.
  2. Any retest should be performed in accordance with the local or national control programme standard.

Boxes with vials each containing 2 mℓ (20 doses).
Boxes with carpoules each containing 1,8 mℓ (18 doses).

Intervet South Africa (Pty) Ltd.
20 Spartan Road, Spartan
1619, RSA
Tel: +27 (0) 11 923 9300
Fax: +27 (0) 11 392 3158

WDT (Wirtschaftsgenossenschaft deutscher Tierärzte e.G)
Serumwerk Memsen
27318 Hoyerhagen

3 April 2007